Polynucleotides for amplification and detection of human beta actin
AssigneeTalis Biomedical Corporation
InventorsJason M. Casolari, Xuewen Jiang, Hédia Maamar
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Abstract
Disclosed herein are primers and probes related to the detection of beta actin [Homo sapiens (human)] via nucleic acid amplification testing (NAAT), for example to amplify and determine the presence of β-actin present in test samples. Specifically, the present disclosure describes primers and probes that bind to the beta actin gene for detection via loop mediated isothermal amplification (LAMP) and molecular beacon hybridization.
Claims8
A composition comprising a set of primers comprising set-29, wherein set-29 comprises primer sequences of SEQ ID NO:
- 132 and SEQ ID NO:
- 133.
A molecular beacon probe comprising a fluorophore and a quencher, wherein the probe sequence comprises SEQ ID NO:
- 184.
A method of detecting β-actin in a test sample, the method comprising:
- (a) extracting nucleic acid from the test sample;
- (b) amplifying a target sequence by reacting nucleic acid extracted in step (a) for less than ten minutes with a reaction mixture comprising a strand displacement DNA polymerase and a sequence-specific isothermal loop mediated amplification (LAMP) primer set;
- and
- (c) detecting the presence or absence of an amplified product of step (b);
- wherein the presence of said amplification product is indicative of the presence of β-actin in the test sample,
- wherein said sequence-specific LAMP primer set is set-29, wherein set-29 comprises primer sequences of SEQ ID NO:
- 132 and SEQ ID NO:
- 133.
Dependencies are inferred from the claim text.