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US 11,891,660 B2Granted
Linked paired strand sequencing
AssigneeSingular Genomics Systems, Inc.
InventorsEli N. Glezer, Martin Maria Fabani, Sabrina Shore, Daan Witters
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Abstract
Provided herein are methods for sequencing both strands of a double stranded nucleic acid fragment that improves fidelity and accuracy of a sequence determination compared to traditional next generation sequencing methods. Compositions and kits for use in the methods are also provided.
Claims20
A method of forming a template polynucleotide on a substrate, said method comprising:
- a) ligating a first end of a double-stranded nucleic acid to a first hairpin adapter comprising a first primer binding sequence, wherein said first adapter is attached to said substrate;
- b) ligating a second adapter to a second end of the double-stranded nucleic acid, wherein the second adapter comprises a second primer binding sequence and a third primer binding sequence, thereby forming said template polynucleotide on said substrate;
- wherein the first primer binding sequence, the second primer binding sequence, and the third primer binding sequence are different.
A method of forming a template polynucleotide on a substrate, said method comprising:
- a) ligating a first end of a double-stranded nucleic acid to a first adapter, wherein said first adapter is attached to said substrate, and wherein said first adapter is a Y-adapter comprising a first primer binding sequence and a second primer binding sequence;
- b) ligating a second adapter to a second end of the double-stranded nucleic acid, wherein the second adapter is a hairpin adapter and comprises a third primer binding sequence, thereby forming said template polynucleotide on said substrate;
- wherein the first primer binding sequence, the second primer binding sequence, and the third primer binding sequence are different.
Dependencies are inferred from the claim text.