Biosensor device and assembly methods
AssigneeQueen Mary University of London
InventorsMatteo Palma, Xinzhao Xu, Pierrick Clement
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Abstract
The invention relates to a method of assembling a biosensor device comprising two or more biosensor units, wherein each unit comprises one or more biosensors comprising one or more carbon nanotubes (CNTs) coated with nucleic acid and one or more sensor molecules coupled to the nucleic acid, wherein each one of the one or more sensor molecules is capable of binding to a target molecule in a sample. Each biosensor unit is capable of detecting a different target molecule in a sample, and each unit comprises one or more biosensors each capable of detecting the same target molecule. The invention further relates to biosensor devices and methods for detecting target molecules in a sample using the same.
Claims29
A method of assembling a biosensor device comprising two or more biosensor units, wherein each unit is capable of detecting a different target molecule in a sample, and wherein each unit comprises one or more biosensors each capable of detecting the same target molecule, the method comprising:
- (i) performing a first cycle to assemble a first biosensor unit comprising:
- A. providing a first solution comprising a population of first carbon nanotubes (CNTs) coated with nucleic acid molecules, wherein nucleic acid molecules are functionalised with functional groups suitable for coupling first sensor molecules to nucleic acid molecules, wherein each first sensor molecule is capable of binding to a first target molecule;
- B. providing a substrate comprising a plurality of electrode pairs;
- C. introducing the first solution to the substrate and immobilising one or more first coated CNTs across a junction separating a first electrode pair, wherein each first coated CNT forms an electrical connection between electrodes of the first electrode pair;
- D. coupling one or more first sensor molecules to the nucleic acid of each first coated CNT, wherein each first sensor molecule is capable of binding to a first target molecule;
- E. washing the entire substrate to remove non-immobilised first coated CNTs;
- and
- (ii) performing a second cycle to assemble a second biosensor unit comprising repeating steps (A) to (E), wherein in repeat step (A) the first solution is replaced with a second solution, the population of first coated CNTs is replaced with a population of second coated CNTs, and wherein functional groups are suitable for coupling second sensor molecules to nucleic acid molecules;
- wherein in repeat step (C) the first solution is replaced with a second solution, the one or more first coated CNTs are replaced with one or more second coated CNTs, and the first electrode pair is replaced with a second electrode pair;
- and wherein in repeat step (D) the one or more first sensor molecules are replaced with one or more second sensor molecules, wherein each second sensor molecule is capable of binding to a second target molecule.
Dependencies are inferred from the claim text.