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US 11,891,648 B2Granted
Method and system for rapidly testing antimicrobial susceptibility
AssigneeBacteromic Sp. Z O.O.
InventorsPawel Debski, Piotr Garstecki
Life of this patent
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Abstract
Disclosed are methods and systems for rapidly testing antimicrobial susceptibility, wherein the qualitative and quantitative susceptibility of an inoculated microorganism against an antimicrobial agent or a combination of antimicrobial agents is determined as a function of one or more slopes of linear trends α of data of readouts.
Claims20
A computer-implemented method for determining the qualitative or quantitative susceptibility of a microorganism inoculum in a phenotypic antimicrobial susceptibility test (AST) using broth dilution, comprising the following steps:
- a) Providing a microorganism inoculum and diluting the inoculum in a medium suitable for broth dilution AST,
- b) Providing a carrier comprising one or more compartments suitable for broth dilution AST, wherein the or at least part of the compartments comprise respectively a single antimicrobial agent or a combination of antimicrobial agents,
- c) Dispensing a sample of the medium containing the diluted inoculum of step a) into the or at least part of the compartments of the carrier of step b) so that the one or more inoculated compartments of the carrier comprise the respective test assays,
- d) Incubating the carrier of step c) comprising the respective one or more test assays,
- e) Measuring, during the incubation step d), at least n times for each of the one or more test assays with constant or inconstant frequency ƒ a signal derived from a chemical or physical property of the inoculated microorganism, wherein the signal represents an essentially monotonic function of the number of the microorganisms in the measured test assay, and reading out corresponding values {xi} at corresponding recording times {ti}, wherein i represents the index number of the measurement represented by an integer 1 to n, and wherein n represents a full integer of 13 or more measurements,
- f) Estimating one or more slopes of linear trends α in data as a function of distribution of a difference from the subtraction of constituents of a pair of the readout values {xi} and {xj}, where j>i, divided by the time interval δtij between taking readouts indexed i and j, whereby part or all of the n readout values {xi}, but at least 13 or more readout values {xi} at corresponding recording times {ti} are used for the distribution, and
- g) Determining the qualitative or quantitative susceptibility of the inoculated microorganism against the single antimicrobial agent or the combination of antimicrobial agents as a function of one or more slopes of linear trends α.
4 more elements
A rapid antimicrobial susceptibility testing system for performing a phenotypic antimicrobial susceptibility test (AST) using broth dilution, the system comprising:
- a) an incubation assembly adapted to house at least one carrier having one or more compartments for receiving a sample of a microorganism inoculum diluted in a growth medium, wherein the or at least part of the compartments comprise respectively a single antimicrobial agent or a combination of antimicrobial agents, so that each inoculated compartment houses a respective test assay, wherein the incubation assembly is configured to provide an incubation and/or measuring environment of the one or more test assays in the one or more compartments,
- b) an interrogation and readout assembly configured to interrogate and readout the one or more test assays during incubation of the one or more carriers, wherein the interrogation and readout assembly is configured to facilitate i) measuring at least n times for each of the one or more test assays, with constant or inconstant frequency ƒ a signal derived from a chemical or physical property of the inoculated microorganism, wherein the signal represents an essentially monotonic function of the number of the microorganisms in the measured test assay, and ii) reading out corresponding values {xi} at corresponding recording times {ti}, wherein i represents the index number of the measurement represented by an integer 1 to n, and wherein n represents a full integer of 13 or more measurements, and
- c) a computing assembly comprising one or more processors and one or more computer-readable media storing instructions that, when executed by the one or more processors, cause the one or more processors to perform operations comprising i) receiving from the interrogation and readout assembly the number n of readouts, the values {xi} and corresponding recording times {ti} and ii) estimating one or more slopes of linear trends α in data as a function of distribution of a difference from a subtraction of constituents of a pair of readout values {xi} and {xj}, where j>i, divided by the time interval δtij between taking readouts indexed i and j, whereby part or all of the n readout values {xi}, but at least 13 or more readout values {xi} at corresponding recording times {ti} are used for the distribution, and iii) determining the qualitative or quantitative susceptibility of the inoculated microorganism against the single anti microbial agent or the combination of antimicrobial agents as a function of one or more slopes of linear trends α.
Dependencies are inferred from the claim text.