Methods, apparatuses, and systems for analyzing complete microorganism strains in complex heterogeneous communities, determining functional relationships and interactions thereof, and identifying and synthesizing bioreactive modificators based thereon

A method, comprising:

• obtaining at least two samples sharing at least one common environmental parameter;
• for an identified sample from the at least two samples detecting the presence of a plurality of microorganism strains;
• determining a total quantity of microorganisms from the plurality of microorganism strains;
• identifying, by sequencing DNA in the identified sample, a set of DNA representing whole genomes corresponding to the plurality of microorganism strains, each DNA representing a whole genome in the set of DNA representing whole genomes corresponding to a microorganism strain from the plurality of microorganism strains in that sample;
• obtaining a set of sequencing reads, from sequencing the DNA in the identified sample, and comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
• determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the identified sample;
• determining an absolute cell count of each microorganism strain in the identified sample from the total quantity of microorganisms from the plurality of microorganism strains included in the microorganism type and the relative frequency of occurrence for each microorganism strain;
• measuring an amount of protein for each microorganism strain by processing the protein in each sample and mapping amino acid sequences from the processing to each DNA representing a whole genome in the set of DNA representing whole genomes;
• generating a gene expression profile associated with each microorganism strain based on the amount of protein associated with the microorganism strain;
• determining an activity for each microorganism strain in the identified sample based on the amount of protein for that microorganism strain;
• identifying active microorganism strains based on the activity for each microorganism strain from the plurality of microorganism strains in the identified sample;
• selecting at least one active microorganism strain from the active microorganism strains based on the gene expression profile associated with each microorganism strain from the active microorganism strains;
• and
• combining the at least one active microorganism strain with a carrier medium to form an active microorganism bioensemble configured to alter a property corresponding to at least one measured metadata when the active microorganism bioensemble is introduced into that target biological environment.

A method, comprising:

• obtaining at least two sample sets, each set having a plurality of samples;
• for an identified sample from a plurality of samples in a sample set, detecting the presence of a plurality of microorganism strains;
• identifying, by sequencing DNA in the identified sample, DNA representing whole genomes of the plurality of microorganism strains;
• obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the identified sample;
• comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
• determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the identified sample;
• determining an absolute cell count of each microorganism strain in each sample from the total quantity of each microorganism type and the relative frequency of occurrence for each microorganism strain;
• measuring RNA for each microorganism strain by sequencing cDNA in each sample and mapping the sequenced cDNA to the set of DNA representing whole genomes;
• generating a gene expression profile identifying expression levels associated with a set of genes associated with each microorganism strain based on the RNA for each microorganism;
• determining an activity for each microorganism strain in the identified sample based on the RNA for each microorganism strain;
• identifying active microorganism strains in each sample, based on the activity for each microorganism strain in the identified sample;
• identifying one or more pathways based on the gene expression profile associated with each microorganism strain from the active microorganism strains;
• and
• subcloning one or more identified pathways for industrial and/or natural product production purposes.

A method of forming a bioreactive modificator based on microorganism strain absolute cell count analysis, the bioreactive modificator configured to alter a property in a target biological environment, comprising:

• obtaining at least two samples sets, each set comprising a plurality of samples;
• for an identified sample from a plurality of samples from a sample set, detecting the presence of a plurality of microorganism strains;
• identifying, by sequencing DNA in each sample, a set of DNA representing whole genomes of the plurality of microorganism strains;
• obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the sample;
• comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between a sequencing read and a DNA representing a whole genome;
• determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the sample;
• determining the absolute cell count of each microorganism strain in each sample from a total quantity of microorganisms and the relative frequency for each microorganism strain;
• measuring RNA for each microorganism strain by sequencing cDNA in each sample and mapping the sequenced cDNA to the DNA representing a whole genome of each microorganism strain;
• determining an activity for each microorganism strain in each sample based on the quantity of cDNA reads mapped to the DNA representing a whole genome of each microorganism strain;
• generating a gene expression profile associated with each microorganism strain based on the RNA associated with each microorganism strain;
• identifying active microorganism strains based on the activity for each microorganism strain;
• identifying genetic similarities between at least two active microorganism strains based on the gene expression profile associated with each microorganism strain from the active microorganism strains to identify at least one functionality of interest;
• selecting at least one compound associated with a metabolic pathway based on the identified at least one functionality of interest;
• and
• combining the selected compound with a carrier medium to form a bioreactive modificator configured to alter a property associated with the at least one metadata of a target biological environment when the bioreactive modificator is introduced into that target biological environment.

A method of forming a bioreactive modificator based on microorganism strain absolute cell count analysis, the bioreactive modificator configured to alter a property in a target biological environment, comprising:

• obtaining at least two sample sets, each set having a plurality of samples;
• for an identified sample from a plurality of samples from a sample set, determining a presence of and total quantity of a plurality of microorganism strains;
• identifying, by sequencing DNA in the identified sample a set of DNA representing whole genomes of the plurality of microorganism strains;
• obtaining a set of sequencing reads associated with the plurality of microorganism strains from sequencing the DNA in the identified sample;
• comparing each sequencing read from the set of sequencing reads with each DNA representing a whole genome in the set of DNA representing whole genomes to identify a match between that sequencing read and a DNA representing a whole genome;
• determining, based on the comparing, a relative frequency of occurrence for each microorganism strain within the identified sample;
• determining the absolute cell count of each microorganism strain in the identified sample from the total quantity of the plurality of microorganism strains and the relative frequency for each microorganism strain;
• measuring RNA for an identified microorganism strain by sequencing cDNA in the identified sample and mapping the sequenced cDNA to the DNA representing a whole genome of the identified microorganism strain;
• determining an activity for the identified microorganism strain in the identified sample based on the RNA for the identified microorganism strain;
• identifying active microorganism strains in the identified sample, based on the activity for each microorganism strain in the identified sample and a measured metadata;
• assigning each strain into one of at least two groups according to predicted function and/or chemistry;
• for each group, identifying genetic similarities between at least two active microorganism strains in the group to identify at least one functionality of interest;
• identifying one or more pathways based on the at least one identified functionality for each group;
• selecting at least one compound based on the identified at least one functionality of interest;
• and
• combining the selected compound with a carrier medium to form a bioreactive modificator configured to alter a property associated with the at least one metadata of a target biological environment when the bioreactive modificator is introduced into that target biological environment.