T cells with reduced surface fucosylation and methods of making and using the same

A method of producing T cells having reduced surface fucosylation, the method comprising:

• culturing T cells in the presence of an effective amount of a fucose analog in a cell culture medium;
• wherein
• said fucose analog is selected from the group consisting of formulae (I) and (II):
• or a pharmaceutically acceptable salt or solvate form thereof, wherein each of formula (I) or (II) can be the alpha or beta anomer or the corresponding aldose form;
• R2 is halogen;
• each of R1, R3, and R4 is independently —OH or a hydrolyzable ester group;
• and R5 is —CH3, or
• each of R1, R2, R3, and R4 is independently —OH or a hydrolyzable ester group;
• and R5 is —C≡CH;
• and
• wherein said T cells cultured in the presence of the effective amount of said fucose analog have at least about 80% reduced surface fucosylation relative to T cells cultured in the absence of said fucose analog;
• and wherein the T cells having reduced surface fucosylation are configured to be used in an adoptive cell therapy.