Methods and systems for monomer chain formation

A method of monomer chain formation comprising:

• immobilizing, using a surface of a waveguide, a monomer chain, wherein the surface of the waveguide further comprises a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent;
• providing a first monomer, wherein the first monomer comprises a capping agent;
• attaching the first monomer to the monomer chain;
• and
• detecting, using a sensor in communication with the waveguide, presence of the first monomer, wherein detecting further comprises:
• introducing a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent is configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte;
• and
• introducing a secondary cleaving agent configured to selectively cleave at least an additional probe as a result of the secondary cleaving agent being in the presence of the analyte.