Biocatalytic techniques
AssigneeHypha Discovery Limited
InventorsJonathan Charles Paul Steele, Antonio De Riso, Francesco Falcioni, Richard Kerry Phipps, Stephen Keith Wrigley, Emily Jade Hopkins, Aksana Rimu Khan, Tetsuo Kokubun, Kinga Linda Nytko, Vincent Poon, Sebastian Schulz, John Maxim Ward, Mariacristina Bawn
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Abstract
A method for the use of a cytochrome P450 enzyme comprising any of SEQ ID NO: 1-118, or mutants thereof or a variant enzyme having at least 70% identity thereto and having CYP450 activity, for the hydroxylation and or dealkylation of an organic compound.
Claims22
An enzyme having cytochrome P450 activity and having at least 90% identity to a cytochrome P450 enzyme comprising SEQ ID NO:
- 29 and comprising at least two of:
- (a) a tryptophan residue at the position corresponding to position 63 of SEQ ID NO:
- 29;
- (b) a tyrosine residue at the position corresponding to position 74 of SEQ ID NO:
- 29;
- and
- (c) an alanine residue at the position corresponding to position 171 of SEQ ID NO:
- 29.
A kit comprising:
- (i) an enzyme having cytochrome P450 activity and having at least 90% identity to a cytochrome P450 enzyme comprising SEQ ID NO:
- 29 and comprising at least two of:
- (a) a tryptophan residue at the position corresponding to position 63 of SEQ ID NO:
- 29;
- (b) a tyrosine residue at the position corresponding to position 74 of SEQ ID NO:
- 29;
- and
- (c) an alanine residue at the position corresponding to position 171 of SEQ ID NO:
- 29;
- or
- (ii) a microorganism that expresses an enzyme as defined in (i);
- or
- (iii) an extract of the microorganism of (ii) comprising the enzyme of (i).
Dependencies are inferred from the claim text.