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US 11,891,641 B2Granted
Optimization of yeast host cells for the production of heterologous proteins
AssigneeLallemand USA, Inc.
InventorsMichelle Oeser, Janet Fisher, Aaron Argyros
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Abstract
The present disclosure concerns yeast host cell especially suited for the expression of heterologous proteins, such as heterologous enzymes. The yeast host cell of the present disclosure exhibits an alteration in the cAMP signaling pathway which allows achieving increased heterologous protein yield and associated biological activity. The yeast host cell can also exhibit polyploidy.
Claims21
A recombinant yeast host cell for making an increased amount of a heterologous protein, wherein the recombinant yeast host cell:
- (i) (a) has a first heterologous nucleic acid encoding the heterologous protein;
- and
- (b) expresses a variant CYR1 protein;
- and/or
- (ii) is obtained by introducing the first heterologous nucleic acid encoding the heterologous protein in an ancestral yeast host cell that expresses a variant CYR1 protein;
- wherein the variant CYR1 protein:
- provides to the recombinant yeast host cell or the ancestral yeast host cell a similar intracellular cAMP production in the presence and in the absence of a cAMP stimulus known to stimulate intracellular cAMP production in a control yeast cell, and
- comprises the amino acid sequence of SEQ ID NO:
- 8 with a substitution at position 869 or a variant thereof having 90% identity with the amino acid sequence of SEQ ID NO:
- 8 with a substitution at position 869.
7 more elements
Dependencies are inferred from the claim text.