Back to results
US 11,891,634 B2Granted
PDE5A destabilizing domains
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsThomas J. Wandless, Ling-Chun Chen, Yusaku Miyamae
Life of this patent
Estimating the term…
Abstract
Disclosed herein are systems, methods, and compositions for rapidly and reversibly destabilizing a target protein in vitro or in vivo, in the presence or absence of a cell-permeable, synthetic molecule or ligand.
Claims2
A conditional protein stability system, comprising:
- a nucleic acid sequence encoding a fusion protein comprising a protein of interest fused in-frame to a single-protein, ligand dependent destabilizing domain wherein the ligand dependent destabilizing domain comprises SEQ ID NO:
- 19 (encoded by SEQ ID NO:
- 36);
- and
- a ligand, wherein the ligand is Sildenafil or Vardenafil,
- wherein upon introduction of the nucleic acid sequence to a cell,
- the fusion protein is expressed, and
- wherein degradation of the protein of interest is decreased upon administration of the ligand.
5 more elements
Dependencies are inferred from the claim text.