Mechanical apparatus and method for isolating stromal vascular fraction

A method for enriching a cell population from a mammalian adipose tissue, the method comprising:

• aspirating adipose tissue into a container assembly using an aspiration tube having a proximal end detachably connected to the container assembly and a distal end inserted into at least one cavity of a mammalian body;
• electrically actuating a mechanical tissue disruption device, while the mechanical tissue disruption device is positioned within the container assembly, with an electrical actuator that is detachably connected to the mechanical tissue disruption device for said electrically actuating, wherein the mechanical tissue disruption device comprises at least one rotatable blade that rotates, when the mechanical tissue disruption device is electrically actuated, to disrupt/rupture the adipose tissue in the container assembly to obtain a cell suspension, wherein the disruption does not utilize an enzyme;
• optionally washing the suspension of cells within the container assembly;
• and
• transferring the container assembly, with the suspension of cells being within the container assembly, into a centrifuge and centrifuging the container assembly to thereby obtain a cell pellet from the suspension of cells within the container assembly, and extracting the cell pellet from within the container assembly, and removing the container assembly from the centrifuge;
• to thereby obtain an enriched cell population.