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US 11,891,626 B2Granted
Isolated discogenic cells, methods of using, and methods of preparing same from mammalian tissue
AssigneeDiscGenics, Inc.
InventorsLara Ionescu Silverman, Kevin T. Foley
Life of this patent
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Abstract
The present disclosure relates to discogenic cell populations, methods of deriving, and methods of using them. The presently described discogenic cell populations may be used to restore or regenerate damaged, diseased, or missing intervertebral discs of a subject. The presently described discogenic cell populations can be derived from and administered or implanted into a subject, or may be derived from an unrelated donor.
Claims30
A discogenic cell population comprising:
- cells derived from mammalian nucleus pulposus disc tissue, wherein the cell population has been passaged at least one time in an anchorage dependent culture, and transferred and maintained in-vitro in anchorage independent culture comprising methylcellulose at a concentration of about 0.1% to about 5% or a low adhesion coating, wherein the cell population, after seven days in anchorage independent culture, expresses at least 2-fold more aggrecan gene and collagen 2 gene than a population of nucleus pulposus cells derived from mammalian disc tissue grown in anchorage dependent culture, and wherein less than about 40% of the cell population expresses the cell surface markers CD24 and CD105.
Dependencies are inferred from the claim text.