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US 11,891,624 B2Granted
Method for the production of thymocyte supernatant
AssigneeHoffmann-La Roche Inc.
InventorsSonja Offner, Markus Horvath, Ingried Saam
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Abstract
Herein is reported a method for producing a thymocyte supernatant comprising the steps of co-cultivating thymocytes and mononuclear cells at a cell ratio of at least 0.5:1.2 in the presence of phorbol-12-myristate-13-acetate and Phytohemagglutinin M for up to 60 hours, and separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.
Claims8
A method for producing a thymocyte supernatant comprising the following steps:
- co-cultivating thymocytes and macrophages at a cell ratio of 0.5:1.2 to 0.5:2 in the presence of phorbol-12-myristate-13-acetate and phytohemagglutinin M for up to 60 hours, and
- separating the co-cultivation medium from the cells and thereby producing the thymocyte supernatant.
Dependencies are inferred from the claim text.