Method for differentiating pluripotent stem cells into desired cell type
AssigneeKeio University
InventorMinoru Ko
Life of this patent
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Abstract
Provided is a method of differentiating a pluripotent stem cell of mammalian origin into a desired cell type by predicting the direction of cell differentiation to be caused by induction of expression of a transcription factor. A human gene expression correlation matrix using human cells has been newly created, and further, it has been confirmed that human pluripotent stem cells can be differentiated into a desired cell type by introducing, into the human pluripotent stem cells, a transcription factor cocktail selected from the matrix.
Claims7
A method of differentiating a pluripotent stem cell into a hepatoblast and/or a liver cell, including a step of introducing a transcription factor including any one of the following (1) to (5) into a pluripotent stem cell of mammalian origin:
- (1) one transcription factor selected from TGIF, TCF4, PITX2, and MEIS1;
- (2) two transcription factors selected from TGIF, TCF4, PITX2, SALL4, and MEIS1;
- (3) three transcription factors selected from TGIF, TCF4, PITX2, SALL4, and MEIS1;
- (4) four transcription factors selected from TGIF, TCF4, PITX2, SALL4, and MEIS1 and
- (5) five transcription factors selected from TGIF, TCF4, PITX2, SALL4, and MEIS1.
Dependencies are inferred from the claim text.