Cell culture media compositions for primary cells
AssigneeMayo Foundation for Medical Education and Research
InventorsAllan B. Dietz, Gaylord Knutson
Life of this patent
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Abstract
Platelet lysate compositions and cell culture media compositions for maintaining and/or growing mammalian cells, such as mammalian endothelial cells (ECs) and mammalian endothelial progenitor cells (EPCs), in particular human ECs (huECs) and human EPCs (huEPCs), such as primary huECs and primary huEPCs, are provided. The cell culture media compositions contain a basal medium, a platelet lysate and, optionally, one or more exogenously added growth factors. Also provided are methods for making and using such cell culture media compositions to grow and/or maintain ECs and EPCs, including huECs and huEPCs, as well as cell culture vessels, dishes, plates, and/or flasks pretreated with the cell culture media compositions.
Claims25
A cell culture medium composition for culturing primary cells, said cell culture medium composition, comprising:
- a basal medium at a concentration of from about 20% (v/v) to about 99% (v/v);
- a lyophilized human platelet lysate reconstituted at a concentration of from about 2.5% (v/v) to about 20% (v/v) and wherein said lyophilized human platelet lysate retains its growth properties after reconstitution;
- an anticoagulant;
- and
- a supplemental exogenous growth factor at a concentration of at least 1 fold to about 2,000 fold of the concentration of the growth factor present in the lyophilized human platelet lysate,
- wherein the cell culture medium composition is free from bulking agents selected from a group consisting of sucrose, mannitol, trehalose and combinations thereof and free from an animal serum, and wherein the cell culture medium composition when culturing primary cells yields at least 80% confluence by 72 hours of the primary cells.
A cell culture medium composition for culturing primary cells, said cell culture medium composition, comprising:
- a basal medium at a concentration of from about 20% (v/v) to about 99% (v/v);
- a lyophilized human platelet lysate reconstituted at a concentration of from about 2.5% (v/v) to about 20% (v/v) and wherein said lyophilized human platelet lysate retains its growth properties after reconstitution;
- and
- a supplemental exogenous growth factor at a concentration of at least 1 fold to about 2,000 fold of the concentration of the growth factor present in the lyophilized human platelet lysate,
- wherein the cell culture medium composition is free from bulking agents selected from a group consisting of sucrose, mannitol, trehalose and combinations thereof, and wherein the cell culture medium composition is free from an animal serum and an anticoagulant.
Dependencies are inferred from the claim text.