Means and methods for producing phosphate containing capsular polysaccharides
AssigneeMedizinische Hochschule Hannover (Mhh)
InventorsTimm Fiebig, Christa Litschko, Rita Gerardy-Schahn, Andrea Bethe, Monika Berger
Life of this patent
Estimating the term…
Abstract
The present invention relates to a host cell, which comprises under the control of a heterologous promoter a polynucleotide comprising a nucleotide sequence encoding a polypeptide capable of synthesizing a polysaccharide consisting of a dimeric repeating unit as well as to a vaccine composition comprising such host cell. Furthermore, either such host cell or a polypeptide expressed by such host cell is used for the production of a polysaccharide consisting of a dimeric repeating unit which may be used as a glycoconjugate vaccine.
Claims15
A host cell, which comprises under the control of a heterologous promoter a polynucleotide comprising a nucleotide sequence encoding a polypeptide, wherein the nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding a polypeptide comprising a TagF-like domain and a GT-B domain having the amino acid sequences shown in SEQ ID NO. 1 and 17, SEQ ID NO. 2 and 18, SEQ ID NO. 3 and 19, SEQ ID NO. 4 and 20, SEQ ID NO. 5 and 21, SEQ ID NO. 6 and 22, SEQ ID NO. 7 and 23, SEQ ID NO. 8 and 24, SEQ ID NO. 9 and 25, SEQ ID NO. 10 and 26, or SEQ ID NO. 11 and 27, wherein the polypeptide synthesizes a polysaccharide consisting of a dimeric repeating unit;
- b) a nucleotide sequence encoding a polypeptide comprising a TagF-like domain and a GT-A domain having the amino acid sequences shown in SEQ ID NO. 12 and 28, SEQ ID NO. 13 and 29, SEQ ID NO. 14 and 30, SEQ ID NO. 15 and 31, or SEQ ID NO. 16 and 32, wherein the polypeptide synthesizes a polysaccharide consisting of a dimeric repeating unit;
- c) a nucleotide sequence encoding a polypeptide having an amino acid sequence that is at least 18% identical to the amino acid sequence of residues 1 to 378 of SEQ ID NO. 3 and that is at least 40% identical to the amino acid sequence of residues 1 to 389 of SEQ ID NO. 23, wherein the polypeptide synthesizes a polysaccharide consisting of a dimeric repeating unit;
- and
- d) a nucleotide sequence encoding a polypeptide having an amino acid sequence that is at least 18% identical to the amino acid sequence of residues 1 to 378 of SEQ ID NO. 3 and that is at least 30% identical to the amino acid sequence of residues 1 to 256 of SEQ ID NO. 32, wherein the polypeptide synthesizes a polysaccharide consisting of a dimeric repeating unit,
- and wherein the nucleotide sequence of (a) to (d) encodes a polypeptide having a conserved tyrosine at a position corresponding to position 49 of SEQ ID NO. 3 or having a conserved aspartic acid at a position corresponding to position 49 of SEQ ID NO. 3.
Dependencies are inferred from the claim text.