Lung bioreactor

A method of providing a wet-matured lung organ, the method comprising:

• providing:
• (i) an organ chamber configured to connect to an arterial line, to a venous line, and to a tracheal line;
• (ii) a chamber pressure sensor connected to the organ chamber, the chamber pressure sensor configured to record and transmit a pressure reading at the organ chamber to a controller;
• (iii) a lung tissue matrix including an airway and substantial vasculature;
• and
• (iv) a media reservoir system;
• connecting the airway to the media reservoir system via the tracheal line;
• connecting the lung tissue matrix to the arterial line and to the venous line;
• connecting the organ chamber to the media reservoir system via an ingress line and an egress line, wherein the ingress line comprises an ingress pump and wherein the egress line comprises a bidirectional egress pump;
• seeding the lung tissue matrix with cells over at least one of the following:
• the arterial line, the venous line, or the tracheal line;
• providing the lung tissue matrix with wet ventilation for a time sufficient for a first desired degree of organ maturation to occur to produce a wet-matured organ;
• and
• maintaining a substantially constant fluid level in the organ chamber during negative pressure wet ventilation, wherein maintaining the substantially constant fluid level comprises:
• monitoring pressure in the organ chamber using the chamber pressure sensor and the controller;
• and
• correcting for fluid shifts through the lung tissue matrix into the organ chamber by adjusting direction and duration of the bidirectional egress pump.