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US 11,891,451 B2Granted
Glycosylation of variable immunoglobulin domains
AssigneesVib Vzw, Universiteit Gent
InventorsNico Callewaert, Bram Laukens, Loes Van Schie, Wander Van Breedam, Wim Nerinckx
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Abstract
The present invention provides nucleotide sequences encoding polypeptides comprising immunoglobulin variable domains with engineered glycosylation acceptor sites. Specifically, the invention provides immunoglobulin variable domain proteins modified with selected glycans and specific glycan-conjugates thereof. Also provided herein are methods for the production of glycosylated immunoglobulin variable domains and glycan-conjugates thereof.
Claims13
A nucleotide sequence encoding a polypeptide, wherein the polypeptide comprises:
- an immunoglobulin single variable domain (ISVD) derived from a camelid heavy chain antibody, wherein the ISVD comprises an amino acid sequence that comprises 4 framework regions (FR) and 3 complementarity determining regions (CDR) according to the following formula (1):
- FR1-CDR1-FR2-CDR2-FR3-CDR3-FR4 (1),
- wherein the ISVD further comprises a N-glycosylation acceptor site present on an amino acid selected from amino acids 83 to 88 of the ISVD according to the AHo numbering convention.
Dependencies are inferred from the claim text.