Methods and compositions for regenerating tissues
AssigneeNellone Therapeutics, Inc.
InventorCymbeline T. Culiat
Life of this patent
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Abstract
Provided are methods for promoting the healing of injuries to tendons and ligaments by administering a NELL1 protein or a nucleic acid encoding a NELL1 protein to a subject in need thereof. Also provided are NELL1 compositions and methods for promoting tissue regeneration, promoting the healing of wounds, and enhancing fibroblast migration, proliferation, or both migration and proliferation.
Claims43
A vector comprising a nucleic acid sequence encoding a NELL1 peptide fragment or variant thereof, having at least 95% sequence identity to SEQ ID NO:
- 17 or 18, wherein said NELL1 peptide fragment or variant thereof has at least one of the properties selected from the group consisting of:
- a) enhanced efficacy in tissue regeneration,
- b) enhanced promotion of wound healing,
- c) easier purification,
- d) higher yield, and
- e) less aggregate formation,
- when compared to the fragment's respective full-length NELL1 protein;
- and wherein said NELL1 peptide fragment or variant thereof lacks the carboxy-terminal 179 amino acid residues of the fragment's respective full-length NELL1 protein.
A recombinant expression cassette comprising a nucleic acid sequence encoding a NELL1 peptide fragment or variant thereof and an operably linked promoter, wherein said NELL1 peptide fragment or variant thereof has at least 95% sequence identity to SEQ ID NO:
- 17 or 18;
- wherein said NELL1 peptide fragment or variant thereof has at least one of the properties selected from the group consisting of:
- a) enhanced efficacy in tissue regeneration,
- b) enhanced promotion of wound healing,
- c) easier purification,
- d) higher yield, and
- e) less aggregate formation,
- when compared to the fragment's respective full-length NELL1 protein;
- and wherein said NELL1 peptide fragment or variant thereof lacks the carboxy-terminal 179 amino acid residues of the fragment's respective full-length NELL1 protein.
A host cell comprising a vector or a recombinant expression cassette, wherein the vector or the recombinant expression cassette comprises a nucleic acid sequence encoding a NELL1 peptide fragment or variant thereof having at least 95% sequence identity to SEQ ID NO:
- 17 or 18;
- wherein said NELL1 peptide fragment or variant thereof has at least one of the properties selected from the group consisting of:
- a) enhanced efficacy in tissue regeneration,
- b) enhanced promotion of wound healing,
- c) easier purification,
- d) higher yield, and
- e) less aggregate formation,
- when compared to the fragment's respective full-length NELL1 protein;
- and
- wherein said NELL1 peptide fragment or variant thereof lacks the carboxy-terminal 179 amino acid residues of the fragment's respective full-length NELL1 protein.
A vector comprising a nucleic acid sequence encoding a NELL1 peptide fragment or variant thereof having at least 99% sequence identity to SEQ ID NO:
- 17 or 18, wherein said NELL1 peptide fragment or variant thereof exhibits enhanced promotion of wound healing when compared to the fragment's respective full-length NELL1 protein.
A recombinant expression cassette comprising a nucleic acid sequence encoding a NELL1 peptide fragment or variant thereof and an operably linked promoter, wherein said NELL1 peptide fragment or variant thereof has at least 99% sequence identity to SEQ ID NO:
- 17 or 18;
- and wherein said NELL1 peptide fragment or variant thereof exhibits enhanced promotion of wound healing when compared to the fragment's respective full-length NELL1 protein.
A host cell comprising a vector or a recombinant expression cassette, wherein the vector or the recombinant expression cassette comprises a nucleic acid sequence encoding a NELL1 peptide fragment or variant thereof having at least 99% sequence identity to SEQ ID NO:
- 17 or 18;
- and wherein said NELL1 peptide fragment or variant thereof exhibits enhanced promotion of wound healing when compared to the fragment's respective full-length NELL1 protein.
Dependencies are inferred from the claim text.