Microcarrier filter bag assemblies and methods of use

A filter bag assembly comprising:

• a flexible first sheet;
• a flexible second sheet overlying and secured to the first sheet so that a compartment is formed therebetween;
• a porous filter sheet disposed between the first sheet and the second sheet so as to divide the compartment into a first compartment and a second compartment;
• a first port secured to the second sheet so as to communicate directly with the first compartment;
• and
• a second port secured to the second sheet so as to communicate directly with the second compartment, the second port being spaced apart from the first port,
• wherein the porous filter sheet is disposed so that fluid entering the first compartment through the first port must pass through the filter sheet before entering the second compartment or exiting through the second port;
• wherein the first sheet and the second sheet each have a first end and an opposing second end;
• and
• wherein the porous filter sheet is disposed between the first sheet and the second sheet at the first ends thereof but is not disposed between the first sheet and the second sheet at the second ends thereof.