Specimen collection container having a fluid separation chamber

A specimen collection container comprising:

• a first chamber configured to collect and maintain a fluid for testing, the first chamber having an open top portion, a sidewall, and a bottom portion at a location that is oppositely disposed from the open top portion;
• a second chamber having a top, a closed bottom, and a sidewall;
• a valve disposed between the first chamber and the second chamber, the valve adjacent the top of the second chamber and the bottom portion of the first chamber and transitionable from an open position which permits fluid communication between the first chamber and the second chamber to a closed position which maintains fluid isolation between the first chamber and the second chamber;
• an expandable material having one surface disposed adjacent to and in contact with_the closed bottom of the second chamber, the expandable material being expandable to transition the valve from the open position to the closed position, such that at the closed position, the valve is located adjacent the top of the second chamber and closes the bottom portion of the first chamber and the one surface of the expandable material remains adjacent to the closed bottom of the second chamber;
• a removable lid adapted to cover the open top portion of the first chamber to prevent the fluid from leaking from the first chamber,
• wherein the fluid for testing is maintained in the first chamber when the valve is in the closed position and the fluid is inaccessible from the first chamber via the valve when the valve is in the closed position:
• and
• a port associated with the first chamber, the port comprising a nozzle defining a channel extending from the first chamber and an exterior of the specimen collection container, the port being transitionable between a closed position, where the fluid is inaccessible, and an open position enabling removal of the fluid from the first chamber.