Burkholderia cenocepacia and Pseudomonas fluorescens compositions and methods of using the same
AssigneeBoard of Trustees of the University of Arkansas
InventorsClemencia M. Rojas, Ruben O. Morawicki, Ines Pinto, Alejandro Rojas
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Abstract
The present invention provides compositions comprising new strains of Pseudomonas fluorescens and Burkholderia cenocepacia. Secreted fraction compositions produced by these bacterial strains, and the methods for producing these compositions are also provided. Herein, the inventors demonstrate that these bacterial strains and compositions may be used to inhibit the growth of a broad spectrum of plant pathogens.
Claims12
A method for inhibiting the growth of Burkholderia glumae on a rice plant comprising contacting the plant with an effective amount of a composition to inhibit the growth of Burkholderia glumae on the plant, wherein the composition is selected from the group consisting of:
- a composition comprising at least 108 CFU per milliliter carrier of a Pseudomonas strain designated as PBL13 and deposited as NRRL Accession No. B-68083, a composition comprising at least 108 CFU per milliliter carrier of a Burkholderia cenocepacia strain designated as PBL18 and deposited as ATCC Accession No. PTA-127637, an agricultural composition comprising a Pseudomonas fluorescens strain designated as PBL13 and deposited as NRRL Accession No. B-68083, an agricultural composition comprising a Burkholderia cenocepacia strain designated as PBL18 and deposited as ATCC Accession No. PTA-127637, and combinations thereof.
A method for inhibiting the growth of Burkholderia glumae on a rice plant, the method comprising:
- a) culturing at least one bacterial strain in a growth medium capable of supporting the growth of the bacterial strain to produce a bacterial culture composition, wherein the bacterial strain is a Pseudomonas strain designated as PBL13 and deposited as NRRL Accession No. B-68083, a Burkholderia cenocepacia strain designated as PBL18 and deposited as ATCC Accession No. PTA-127637, or a combination thereof,
- b) separating the bacterial strain from the bacterial culture composition to produce a cell-free composition;
- and
- c) contacting the plant with an effective amount of the cell-free composition to inhibit the growth of Burkholderia glumae on the plant.
Dependencies are inferred from the claim text.