Organic light-emitting diode display substrate and manufacturing method thereof, and display panel

An organic light-emitting diode display substrate having a plurality of pixel regions, comprising a base substrate, and a pixel defining layer disposed on the base substrate;

• wherein, in regions of the pixel defining layer, which correspond to the pixel regions, there are disposed accommodation parts which penetrate the pixel defining layer in a direction perpendicular to the base substrate, the pixel defining layer is further provided with guide parts disposed corresponding to the accommodation parts,
• the guide parts are located on a periphery of the corresponding accommodation parts and are formed by recessed areas which are formed at positions on a side of the pixel defining layer away from the base substrate, the recessed areas do not penetrate the pixel defining layer in the direction perpendicular to the base substrate, and
• an orthographic projection of the guide part on the base substrate is directly coupled to an orthographic projection of the corresponding accommodation part on the base substrate.