Optics, device, and system for assaying and imaging
AssigneeEssenlix Corporation
InventorsStephen Y. Chou, Wei Ding, Ji Qi, Yuecheng Zhang, Wu Chou, Mingquan Wu, Xing Li, Jun Tian
Life of this patent
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Abstract
A method of assaying an analyte in a sample is disclosed. The method includes having a sample holder with a sample contact area for contacting a sample with an analyte, having a plurality of calibration structures on the sample contact area of the sample holder, imaging a part of the sample contact area that has the calibration structures, and using an algorithm that includes an image, calibration structures in the image, and artificial intelligence and/or machine learning to identify the analyte and/or determine the analyte concentration.
Claims32
A method of assaying an analyte in a sample comprising:
- (i) having a sample holder with a sample contact area for contacting a sample containing or suspected of containing an analyte;
- (ii) having a plurality of calibration structures in the sample contact area of the sample holder, wherein the calibration structures have definite and predetermined geometry, and each of the calibration structures has an average lateral dimension of 100 μm or less;
- (iii) imaging a part of the sample contact area that has the calibration structures with an imager to obtain an image;
- and
- (iv) using an algorithm that comprises (a) the image in (iii), (b) the calibration structures in the image, and (c) machine learning to (1) identify the analyte, or (2) determine the analyte concentration, or (3) both (1) and (2).
A system of assaying an analyte in a sample comprising:
- (i) a sample holder with a sample contact area for contacting a sample containing or suspected of containing an analyte;
- (ii) a plurality of calibration structures on the sample contact area of the sample holder, wherein the calibration structures have predetermined inter-distance between two neighboring calibration structures and each of the calibration structures has a lateral dimension of an average 100 μm or less;
- (iii) an imager that images a part of the sample contact area that has the calibration structures;
- and
- (iv) a non-transitory computer-readable medium comprising an algorithm that comprises (a) the image in (iii), (b) the calibration structures in the image, and (c) machine learning, to (1) identify the analyte, or (2) determine the analyte concentration or both (1) and (2).
An optical adaptor for assaying an analyte in a sample, comprising:
- a sample holder frame and an imager, wherein:
- (i) the sample holder frame has a sample holder and at least one plate with a sample contact area for contacting a sample containing or suspected of containing an analyte;
- and
- (ii) the sample holder on the sample contact area has a plurality of calibration structures that have predetermined and known inter-distance between two neighboring calibration structures, and wherein the optical calibration structures are imaged by an imager and are used by an algorithm that comprises (a) the image from the imager, (b) the calibration structures in the image, and (c) machine learning, to (1) identify the analyte, or (2) determine the analyte concentration, or both (1) and (2).
Dependencies are inferred from the claim text.