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AssigneeWestlake University
InventorsLiang Gao, Bi-Chang Chen
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A lattice light sheet microscope includes a single spatial light modulator (SLM), the optical modulation plane of which conjugates to an image plane of an excitation objective and which is configured to: generate an optical lattice by loading a phase map obtained from a central cross section of a corresponding optical lattice of a desired lattice light sheet, and tile the optical lattice by loading a phase map obtained from an off-center cross section of the corresponding optical lattice. The lattice light sheet microscope also includes a transparent annular diaphragm arranged at a plane conjugating to the entrance pupil of the excitation objective lens. The lattice light sheet microscope further includes a first galvanometer configured to: scan each optical lattice in the extending direction thereof so as to form a tiled lattice light sheet.
A lattice light sheet microscope which comprises a spatial light modulator, an excitation objective, a transparent annular diaphragm, and a first galvanometer, wherein
A method for tiling a lattice light sheet in a lattice light sheet microscope, wherein the lattice light sheet microscope comprises a spatial light modulator, an excitation objective, a transparent annular diaphragm and a first galvanometer, wherein an optical modulation plane of the spatial light modulator is conjugated to an image plane of the excitation objective and the spatial light modulator is configured to modulate illumination light, wherein the transparent annular diaphragm is arranged at a plane conjugated to an entrance pupil of the excitation objective to filter illumination light modulated by the spatial light modulator, and wherein the spatial light modulator is a single spatial light modulator, wherein the method comprises:
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