Apparatuses and methods for multi-direction digital scanned light sheet microscopy
AssigneeUniversity of Washington
InventorsAdam K. Glaser, Jonathan T. C. Liu
Life of this patent
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Abstract
Embodiments disclosed herein directed to multi-direction digital light sheet microscopy (mDSLM). In mDSLM a sample may be illuminated with an illumination sheet which has a first angular diversity along a first axis and a second angular diversity along a second axis which is orthogonal to the first axis. An mDSLM system includes beam shaping optics which may passively reshape light into the illumination sheet. In some embodiments, scanning optics may be used to scan the illumination sheet with respect to the sample. In some embodiments, a confocal aperture may be used to limit out of focus light which reaches a detector of the mDSLM system.
Claims24
An apparatus comprising:
- a light source configured to emit a beam of light;
- illumination optics configured to provide an illumination sheet based on the beam of light and illuminate a sample with the illumination sheet, the illumination sheet having a first angular diversity along a first axis and a second angular diversity along a second axis orthogonal to the first axis, wherein the first angular diversity is greater than the second angular diversity, wherein the illumination sheet has a first numerical aperture along the first axis and a second numerical aperture along the second axis and wherein the first numerical aperture is greater than the second numerical aperture;
- and
- collection optics configured to receive light from the sample along the second axis.
A method comprising:
- directing an illumination sheet at a sample, the illumination sheet comprising a first angular diversity along a first axis and a second angular diversity along a second axis orthogonal to the first axis, wherein the first angular diversity is greater than the second angular diversity, wherein the illumination sheet has a first numerical aperture along the first axis and a second numerical aperture along the second axis, and wherein the first numerical aperture is greater than the second numerical aperture;
- and
- receiving light from the sample, wherein the received light is based on the illumination sheet.
Dependencies are inferred from the claim text.