Methods and devices for analyte collection, extraction, concentration, and detection for clinical applications

A method of concentrating an analyte using an aqueous two-phase system, said method comprising:

• providing a porous matrix comprising salt-polymer aqueous two-phase system (ATPS) comprising a mixed phase solution that separates into a first phase solution and a second phase solution as said ATPS moves through said porous matrix wherein said ATPS is provided as a dehydrated salt and a dehydrated polymer that when rehydrated form said mixed phase of an aqueous two-phase system, where said dehydrated salt is disposed in said porous matrix upstream from said dehydrated polymer;
• applying a sample solution comprising said analyte to said porous matrix where said sample solution rehydrates the dehydrated salt and dehydrated polymer to form the mixed phase solution that separates into a first phase solution and a second phase solution as said ATPS moves through said porous matrix and concentrates the analyte into the first phase solution or the second phase solution or an interface between the first phase solution and second phase solution.