Method for predicting resistance

A method for obtaining gametes from rainbow trout having increased resistance to infectious pancreatic necrosis (IPN), said method comprising:

• a) providing an initial population of rainbow trout;
• b) obtaining a nucleic acid sample from at least one individual rainbow trout within said initial population;
• c) detecting in each of said nucleic acid samples the presence of at least one allele conferring IPN resistance (“IPN resistance allele”) within the genome of said rainbow trout, wherein the at least one IPN resistance allele is an allele of at least one single nucleotide polymorphism (SNP) selected from the SNPs listed in the following table:
• Position in
• IPN
• Non-IPN
• SNP
• Name
• SEQ ID
• GenBank
• GenBank
• dbSNP ss-
• resistance
• resistance
• #
• Affymetrix ID
• NO:
• Contig
• Contig
• No (SS#)
• allele
• allele
• 1
• AX-89929954
• 1
• FR904293.1
• 1651243
• 1398298005
• C
• A
• 2
• AX-89918280
• 2
• FR904293.1
• 1353665
• 1399389616
• G
• A
• 4
• AX-89960828
• 4
• FR932837.1
• 3160
• 1399779599
• T
• C ;
• d) selecting a rainbow trout from said initial population based on the presence of the at least one IPN resistance allele in the nucleic acid samples;
• and
• e) isolating gametes from said selected trout.

A method for obtaining rainbow trout having increased resistance to infectious pancreatic necrosis (IPN), said method comprising:

• a) providing an initial population of rainbow trout;
• b) obtaining a nucleic acid sample from at least one individual rainbow trout within said initial population;
• c) detecting in each of said nucleic acid samples the presence of at least one allele conferring IPN resistance (“IPN resistance allele”) within the genome of said rainbow trout, wherein the at least one IPN resistance allele is an allele of at least one single nucleotide polymorphism (SNP) selected from the SNPs listed in the following table:
• Position in
• IPN
• Non-IPN
• SNP
• Name
• SEQ ID
• GenBank
• GenBank
• dbSNP ss-
• resistance
• resistance
• #
• Affymetrix ID
• NO:
• Contig
• Contig
• No (SS#)
• allele
• allele
• 1
• AX-89929954
• 1
• FR904293.1
• 1651243
• 1398298005
• C
• A
• 2
• AX-89918280
• 2
• FR904293.1
• 1353665
• 1399389616
• G
• A
• 4
• AX-89960828
• 4
• FR932837.1
• 3160
• 1399779599
• T
• C ;
• d) selecting a rainbow trout from said initial population based on the presence of the at least one IPN resistance allele in the nucleic acid samples;
• e) isolating gametes from said selected trout;
• and
• f) utilizing said isolated gametes to produce progeny rainbow trout comprising increased resistance to IPN.