Primer compositions for detecting thalassemia
AssigneeImam Abdulrahman Bin Faisal University
InventorsJ. Francis Borgio, Sayed Abdulazeez, Fahd A. Al-Muhanna, Amein Kadhem Al-Ali
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Abstract
One aspect of the invention is a method for amplifying alpha globin genes HBA1, HBA2 and HBA12 in a single PCR tube to determine an HBA genotype of a subject. This method employs five primers selected to accurate and sensitively identify the HBA1, HBA2, and HBA12, a gene found at a higher frequency in citizens of Saudi Arabia, by accurately annealing to nucleic acids in a biological sample and simultaneously amplifying sequences encoding the alpha globin genes. This invention includes a procedure and required reagents for the amplification of alpha globin genes in a single PCR tube.
Claims17
A primer pool composition, comprising:
- a set of primers for each of HBA1, HBA2 and HBA12,
- wherein the set of HBA1 primers comprises MA1F (SEQ ID NO:
- 3) and MA12R (SEQ ID NO:
- 4),
- the set of HBA2 primers comprises MA2F (SEQ ID NO:
- 5) and MA2R (SEQ ID NO:
- 2 or 6) and
- the set of HBA12 primers comprises MA12F (SEQ ID NO:
- 1) and MA2R (SEQ ID NO:
- 2 or 6);
- wherein at least one of said HBA1, HBA2 or HBA12 primers in the primer pool composition has been modified by conjugation to a fluorescent tag, biotin, quencher or other detectable reporter moiety and/or by substitution of a chemically modified, non-natural nucleotide for at least one natural nucleotide.
A primer pool composition comprising at least two primer pairs selected from the group consisting of a set of primer pairs for HBA1, HBA2 and HBA12,
- wherein the set of HBA1 primers comprises MA1F (SEQ ID NO:
- 3) and MA12R (SEQ ID NO:
- 4),
- the set of HBA2 primers comprises MA2F (SEQ ID NO:
- 5) and MA2R (SEQ ID NO:
- 2 or 6) and
- the set of HBA12 primers comprises MA12F (SEQ ID NO:
- 1) and MA2R (SEQ ID NO:
- 2 or 6);
- wherein at least one of said HBA1, HBA2 or HBA12 primers in the primer pool composition has been modified by conjugation to a fluorescent tag, biotin, quencher or other detectable reporter moiety and/or by substitution of a chemically modified, non-natural nucleotide for at least one natural nucleotide.
Dependencies are inferred from the claim text.