Polynucleotide based movement, kits and methods related thereto
AssigneeEmory University
InventorsKhalid Salaita, Kevin Yehl
Life of this patent
Estimating the term…
Abstract
This disclosure relates DNA based movement of objects. In certain embodiments, particles, pairs of particles, or rods are conjugated with single stranded DNA that hybridizes to a single stranded RNA that is conjugated to a substrate. When the DNA particle, pair of particles, or rod interacts with the surface RNA in the presence of an endonuclease, such as RNase H and the DNA hybridizes to the RNA, then the particle, pair of particles, or rod moves along the surface. The complementarity of the DNA and RNA affect the velocity.
Claims19
A method for moving a particle comprising DNA, comprising:
- providing a rolling particle system comprising,
- a spherical particle comprising a coating of single stranded DNA;
- a planar substrate comprising a coating of single stranded RNA, wherein the RNA density is between about 2×104 molecules molecules/μm2 and about 6×104 molecules/μm2, wherein the RNA is conjugated to a fluorescent molecule, wherein the substrate further comprises polyethylene glycol;
- and
- RNase H;
- and
- placing the single stranded DNA coated particle on the surface of the planar substrate in the presence of RNase H such that the particle moves on the surface of the substrate.
A method for moving a rod comprising DNA, comprising:
- providing a rolling particle system comprising,
- a rod of two or more spherical particles comprising a coating of single stranded DNA;
- a planar substrate comprising a coating of single stranded RNA, wherein the RNA density is between about 2×104 molecules molecules/μm2 and about 6×104 molecules/μm2, wherein the RNA is conjugated to a fluorescent molecule, wherein the substrate further comprises polyethylene glycol;
- and
- RNase H;
- placing the single stranded DNA coated particle on the surface of the planar substrate in the presence of RNase H such that the rod moves on the surface of the substrate.
Dependencies are inferred from the claim text.