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US 11,884,965 B2Granted
Chimeric primers with hairpin conformations and methods of using same
AssigneeLuminex Corporation
InventorsDouglas F. Whitman, Hongwei Zhang
Life of this patent
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Abstract
Methods and compositions for nucleic acid amplification, detection, and genotyping techniques are disclosed. In one embodiment, a nucleic acid molecule having a target-specific primer sequence; an anti-tag sequence 5′ of the target-specific primer sequence; a tag sequence 5′ of the anti-tag sequence; and a blocker between the anti-tag sequence and the tag sequence is disclosed. Compositions containing such a nucleic acid molecule and methods of using such a nucleic acid molecule are also disclosed.
Claims15
A method for amplifying a target nucleic acid comprising:
- (a) providing a first primer pair comprising:
- (i) a first primer comprising:
- a first target-specific primer sequence;
- an anti-tag sequence 5′ of the target-specific primer sequence;
- a tag sequence 5′ of the anti-tag sequence;
- and
- a blocker between the anti-tag sequence and the tag sequence;
- and
- (ii) a second primer comprising:
- a second target-specific primer sequence;
- and
- (b) providing a reporter;
- (c) providing a capture complex comprising an anti-tag sequence attached to a solid support;
- and
- (d) amplifying the target nucleic acid by combining the first primer pair, the reporter, the capture complex, and a sample comprising the target nucleic acid under conditions suitable for amplification of the target nucleic acid.
12 more elements
Dependencies are inferred from the claim text.