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US 11,884,955 B2Granted
Solubilization of MSW with blend enzymes
AssigneeRenescience A/S
InventorsHanne Risbjerg Soerensen, Lisa Rosgaard, Henrik B Nielsen, Lone Baekgaard, Joanna Wawrzynczyk
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Abstract
The present invention relates to a method for solubilisation or hydrolysis of Municipal Solid Waste (MSW) with an enzyme blend and an enzyme composition for solubilization of Municipal Solid Waste (MSW), the enzyme composition comprising a cellulolytic background composition and a protease, lipase and/or beta-glucanase.
Claims19
A process for solubilizing waste comprising:
- contacting Municipal Solid Waste (MSW) with an enzyme composition comprising a cellulolytic background composition comprising (a) a cellobiohydrolase I or variant thereof;
- (b) a cellobiohydrolase II or variant thereof;
- (c) a beta-glucosidase or variant thereof;
- and (d) a polypeptide having cellulolytic enhancing activity, wherein the cellulolytic background composition is present in the enzyme composition at a ratio between 40%-99% w/w;
- and one or more enzymes selected from (i) a protease at a ratio between about 5-15% w/w of the total enzyme protein;
- ii) a lipase at a ratio between about 2.5-7.5% w/w of the total enzyme protein;
- and/or iii) a beta-glucanase at a ratio between about 10-20% w/w of the total enzyme protein;
- and wherein the enzyme composition further comprises one or more enzymes selected from (iv) a pectate lyase;
- (v) a mannanase;
- and (vi) an amylase.
7 more elements
A process for solubilizing waste comprising:
- contacting Municipal Solid Waste (MSW) with an enzyme composition comprising a cellulolytic background composition comprising (a) a cellobiohydrolase I or variant thereof;
- (b) a cellobiohydrolase II or variant thereof;
- (c) a beta-glucosidase or variant thereof;
- and (d) a polypeptide having cellulolytic enhancing activity;
- and further comprising a i) a protease at a ratio between about 5-15% w/w of the total enzyme protein;
- ii) a lipase at a ratio of between about 2.5-7.5% w/w of the total enzyme protein;
- and/or
- iii) a beta-glucanase at a ratio between about 10-20% w/w of the total enzyme protein,
- wherein the amount of enzyme composition to MSW is about 0.5 to about 50 mg per gram of MSW.
6 more elements
Dependencies are inferred from the claim text.