Genetically modified organisms for production of polyketides
AssigneePyrone Systems, Inc.
InventorsAlexander Hutagalung, Jose Miguel Laplaza
Life of this patent
Estimating the term…
Abstract
Strains of yeasts are provided containing the genes for the production of cannabinoids from fatty acids. The enzymes that mediate cannabinoid production are localized to the cytosol, peroxisome or different compartments within the secretory pathway (e.g., endoplasmic reticulum, Golgi, vacuole) to ensure efficient production. The engineered microorganisms produce cannabinoids in a controlled fermentation process.
Claims20
A method of producing a polyketide in a peroxisome, the method comprising:
- providing a fatty acid to a genetically modified microorganism, wherein the genetically modified microorganism is a yeast or a fungus, wherein the microorganism comprises a biosynthetic enzyme targeted to the peroxisome in the microorganism, wherein the biosynthetic enzyme is capable of catabolizing the fatty acid into the polyketide, wherein the biosynthetgic enzyme is an acyl-CoA oxidase, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, beta-ketothiolase, thiolase, acyl-CoA synthase, polyketide synthase, cannabidiolic acid synthase, tetrahydrocannabidiolic acid, olivetolic acid synthase, tetraketide synthase, TKS 1, or TKS 1 p;
- culturing the microorganism under conditions sufficient to produce the polyketide in the peroxisome;
- and
- isolating the polyketide.
A genetically modified microorganism, wherein the genetically modified microorganism is a yeast or a fungus, comprising a biosynthetic enzyme targeted to a peroxisome in the microorganism, wherein the biosynthetic enzyme is capable of catabolizing a fatty acid into a polyketide, wherein the biosynthetic enzyme is an acyl-CoA oxidase, enoyl-CoA hydratase, 3-hydroxyacyl-CoA dehydrogenase, beta-ketothiolase, thiolase, acyl-CoA synthase, polyketide synthase, cannabidiolic acid synthase, tetrahydrocannabidiolic acid, olivetolic acid synthase, tetraketide synthase, TKS1, or TKS 1p.
A genetically modified microorganism, wherein the genetically modified microorganism is a yeast or a fungus, comprising a biosynthetic enzyme targeted to a peroxisome in the microorganism, wherein the biosynthetic enzyme is capable of catabolizing a fatty acid, vegetable oil, or an alkane into a polyketide, wherein the biosynthetic enzyme comprises a peroxisomal targeting sequence having a consensus sequence of [S/A/H/C/E/P/Q/V]-[K/R/H/QHL/F1 as set forth in SEQ ID NO:
- 7, or GRRAKL as set forth in SEQ ID NO:
- 6.
Dependencies are inferred from the claim text.