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US 11,884,946 B2Granted
Generation of stable protein films using fluorous solvent
AssigneeUniversity of Massachusetts
InventorsLi-Sheng Wang, Sanjana Gopalakrishnan, Vincent M. Rotello
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Abstract
Methods of preparing protein films in the presence of organofluorine compounds are provided that can produce protein films that retain the solution phase characteristics of the proteins. The protein films can be coatings for medical devices.
Claims17
A method of making a protein film on a substrate, the method comprising:
- depositing a protein solution comprising one or more proteins on a substrate, to form the protein film on the substrate, wherein the one or more proteins are a fibrous protein, a globular protein, a transmembrane transport protein, a hormone or growth factor protein, a receptor protein, a DNA-binding protein, a transcription regulation protein, an immune system protein, a nutrient storage or transport protein, a chaperone protein, an enzyme, or a combination thereof;
- immersing the protein film on the substrate in at least one organofluorine compound in liquid form that is chemically inert to the one or more proteins, and heating the at least one organofluorine compound to stabilize the protein film, wherein the stabilized protein film on the substrate retains the secondary structure of the one or more proteins in the protein solution;
- removing the protein film on the substrate from immersion in the at least one organofluorine compound;
- and
- washing the protein film on the substrate to remove any residual at least one organofluorine compound present following removal,
- wherein the protein film on the substrate following removal from the immersion is a hydrophilic protein film and is free of any organofluorine compound and the organofluorine compound is optionally preheated ahead of immersion.
3 more elements
A method of making a protein film on a substrate, the method comprising:
- depositing a protein solution comprising anionic bovine serum albumin on the substrate, to form a protein film on the substrate;
- dipping the protein film on the substrate in perfluoroperhydrophenanthrene (PFHP) and heating the PFHP while the substrate is immersed therein to maintain a temperature of about 140° C. to about 200° C. for about 1 to about 30 minutes, to stabilize the protein film on the substrate;
- removing the protein film on the substrate from immersion in the PFHP;
- and
- washing the protein film on the substrate to remove any residual at least one organofluorine compound present following removal,
- wherein the protein film on the substrate is a hydrophilic protein film and is free of the PFHP following the removal of the protein film on the substrate from the PFHP.
3 more elements
Dependencies are inferred from the claim text.