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US 11,884,943 B2Granted
Engineered Pyrococcus enzymes and uses thereof
AssigneeSingular Genomics Systems, Inc.
InventorsSouad Naji, Eli N. Glezer, Youngjin Cho, Zachary Terranova, Abrehet Abdu
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Abstract
Provided herein are modified Archaeal family B polymerases derived from species of the Archaeal microorganism Pyrococcus that exhibit improved incorporation of nucleotide analogues utilized in DNA sequencing.
Claims19
A polymerase comprising an amino acid sequence that is at least 80% identical to a continuous 500 amino acid sequence within SEQ ID NO:
- 1;
- comprising the following amino acids:
- an alanine at amino acid position 409 or an amino acid position functionally equivalent to amino acid position 409;
- a glycine at amino acid position 410 or an amino acid position functionally equivalent to amino acid position 410;
- a proline at amino acid position 411 or an amino acid position functionally equivalent to amino acid position 411;
- and
- a glutamine, arginine, or alanine at amino acid position 93 or an amino acid position functionally equivalent to amino acid position 93.
4 more elements
A polymerase comprising an amino acid sequence that is at least 80% identical to a continuous 500 amino acid sequence within SEQ ID NO:
- 1, comprising a first mutation at amino acid position 409 or an amino acid position functionally equivalent to amino acid position 409, and at least one mutation at
- amino acid position 429 or an amino acid position functionally equivalent to amino acid position 429,
- amino acid position 443 or an amino acid position functionally equivalent to amino acid position 443,
- amino acid position 507 or an amino acid position functionally equivalent to amino acid position 507, or
- amino acid position 510 or an amino acid position functionally equivalent to amino acid position 510.
2 more elements
Dependencies are inferred from the claim text.