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US 11,884,942 B2Granted
Compositions and methods comprising the use of Exiguobacterium acetylicum and Bacillus coagluans α-glucanotransferase enzymes
AssigneeDupont Nutrition Biosciences Aps
InventorSlavko Kralj
Life of this patent
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Abstract
An isolated and/or purified α-glucanotransferase from Exiguobacterium acetylicum, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the α-glucanotransferase and variants thereof, host cells comprising the synthetic nucleic acids, and compositions comprising the α-glucanotransferase are provided. Methods of using the compositions include the manufacture of oligosaccharides.
Claims16
A composition comprising at least:
- (i) an isolated GH70 subfamily 4 α-glucanotransferase comprising an amino acid sequence that is at least 95% identical to amino acids 33-736 of SEQ ID NO:7 or amino acids 22-726 of SEQ ID NO:11, and
- (ii) a glucooligosaccharide with α-(1→4) and α-(1→6) glycosidic linkages.
Dependencies are inferred from the claim text.