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US 11,884,941 B2Granted
Non-canonical lipoproteins with programmable assembly and architecture and method of making non-canonical lipoproteins
AssigneeSyracuse University
InventorDavoud Mozhdehi
Life of this patent
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Abstract
Artificial lipoproteins bearing non-canonical post-translational modifications that are synthesized by leveraging substrate promiscuity of an acyltransferase. The non-canonical functionality of these lipoprotein results in a distinctive hysteretic assembly that is absent from the canonical lipoproteins and is used to prepare hybrid multiblock materials with precise and programmable patterns of amphiphilicity due to the unique assembly and function of the non-canonical post-translational modifications.
Claims8
A method of synthesizing a non-naturally occurring lipoprotein, comprising the steps of:
- modifying a host organism to express a Saccharomyces cerevisiae N-myristoyl transferase;
- modifying the host organism to express an intrisincally disordered peptide polymer fused to a peptide substrate of the N-myristoyl transferase;
- and
- coexpressing the N-myristoyl transferase and the intrinsically disordered peptide polymer from the host organism in the presence of an expression media having a non-natural fatty acid having the formula R—COOH, where R contains a linear chain of no more than 15 atoms of C, N, O or S, so that the intrinsically disordered peptide polymer fused to the peptide substrate is subjected to post-translational modification by the host organism to form a non-naturally occurring lipoprotein.
1 more element
Dependencies are inferred from the claim text.