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US 11,884,937 B2Granted
Activation of innate immunity for enhanced nuclear reprogramming of somatic cells with mRNA
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsJohn P. Cooke, Eduard Yakubov
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Abstract
The nuclear reprogramming of somatic cells with mRNA encoding reprogramming factors is shown to be greatly accelerated by activation of innate immune responses in the somatic cell. Methods of activating innate immunity include activation of PKR, of toll-like receptors, e.g. TLR3, etc. In some embodiments the mRNA provides the activator of innate immunity.
Claims12
A method of nuclear reprogramming of mammalian somatic cells, the method comprising:
- contacting a population of the mammalian somatic cells with:
- an effective dose of an innate immune response activator;
- and
- a population of modified mRNA (mmRNA), wherein at least 1% of the mmRNA population comprises 5′ triphosphate encoding a cocktail of reprogramming or transcriptional factors.
1 more element
Dependencies are inferred from the claim text.