Methods and compositions for T cell activation
AssigneeWashington University
InventorsEynav Klechevsky, Amit Pathak, Bapi Sarker
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Abstract
Among the various aspects of the present disclosure is the provision of methods, synthetic DC, and compositions for T cell activation. The present disclosure provides for synthetic dendritic cells (DCs), methods of generating synthetic dendritic cells (DCs), methods of generating T cell-encapsulated gelatin microspheres and microcapsules, methods of activating T cells using synthetic DCs, methods for expanding T cells against individualized antigen-specific mutational antigens using synthetic DCs, and methods of treating a chronic disease (e.g., HIV, HPV) or cancer using the synthetic DCs.
Claims13
A synthetic dendritic cell (synthetic DC) comprising:
- a delivery agent, wherein the delivery agent is a hydrogel substrate comprising an oxidized alginate-gelatin covalently cross-linked (ADA-GEL) hydrogel;
- a linker;
- a binding moiety;
- and
- at least one T cell activation agent;
- wherein the linker is coupled to the delivery agent, the binding moiety is coupled to the linker, and the T cell activation agent is coupled to the binding moiety;
- and
- wherein the synthetic DC is capable of initiating activation and expansion of T cells.
A method of generating a synthetic dendritic cell (DC) comprising:
- (i) providing a hydrogel delivery agent comprising an oxidized alginate-gelatin covalently cross-linked (ADA-GEL) hydrogel;
- (ii) providing a linker;
- (iii) providing a binding moiety;
- (iv) providing one or more T cell activation agents;
- (v) coupling the linker to the hydrogel delivery agent;
- (vi) coupling the linker to the binding moiety;
- and
- (vii) coupling the binding moiety to the one or more T cell activation agents.
Dependencies are inferred from the claim text.