Supported in vitro developed tissue culture and culturing methods
AssigneeImba—Institut FüR Molekulare Biotechnologie GmbH
InventorsJürgen Knoblich, Madeline A. Lancaster
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Abstract
An elongated or fiber-supported multicellular aggregation of multipotent cells, wherein multipotent cells are arranged in an oblong or longish arrangement with an aspect ratio of a prolate dimension to a perpendicular dimension of at least 2:1, or supported by a fibrous structure, and wherein the aggregate contains cells at different stages of differentiation, and the aggregate contains polar cells; methods of generating such aggregates; methods of developing the aggregates further into tissue organoids and kits for such methods.
Claims25
A fiber-supported multicellular aggregation of neural lineage with neuronal differentiated cells wherein cells are arranged on a fibrous structured support, wherein said support is a biocompatible polymer that is not a biopolymer or wherein said fibrous structured support is a protein, and said support consists of 1 to 50 fibers, and wherein said aggregate contains cells at different stages of differentiation, including polar cells, the polar cells having a uniform orientation;
- and wherein said multicellular aggregation is having a size of at least 50 μm.
An elongated multicellular aggregation of neural lineage with neuronal differentiated cells wherein cells are arranged in an oblong or longish arrangement with an aspect ratio of a prolate dimension to a perpendicular dimension of at least 2:1, and wherein the cells are arranged on a support consisting of 1 to 50 fibers;
- including polar cells, the polar cells having a uniform orientation;
- and wherein said multicellular aggregation is having a size of at least 50 μm.
Dependencies are inferred from the claim text.