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US 11,884,932 B2Granted
In vitro production of medial ganglionic eminence precursor cells
AssigneeThe Regents of the University of California
InventorsCory R. Nicholas, John L. Rubenstein, Arnold R. Kriegstein, Arturo Alvarez-Buylla
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Abstract
Methods and systems for generating MGE precursor cells in vitro as well as compositions of enriched MGE precursor cells are provided. The methods and systems provide efficient production of MGE precursors. The methods and systems disclosed herein provide functional MGE precursors which differentiate into functional GABAergic interneurons.
Claims27
A method comprising:
- a) providing primate pluripotent stem (pPS) cells in a culture medium;
- b) introducing to the culture medium, factors comprising:
- i) an activator of the sonic hedgehog (shh) pathway,
- ii) a SMAD inhibitor, and
- iii) a wnt pathway inhibitor,
- to produce a cell culture enriched in MGE precursor cells;
- c) culturing the MGE precursor cells to produce PSA-NCAM-negative or DLX2-negative cells;
- and
- d) enriching for the PSA-NCAM-negative or DLX2-negative cells.
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Dependencies are inferred from the claim text.