CPMV enhancer elements
AssigneeMedicago Inc.
InventorsPierre-Olivier Lavoie, Marc-Andre D'Aoust
Life of this patent
Estimating the term…
Abstract
An expression enhancer comprising a CPMV 5′UTR nucleotide sequence consisting of X nucleotides (CMPVX), where X=160, 155, 150, or 114 of SEQ ID NO:1, or consisting of a nucleotide sequence comprising from about 80% to 100% sequence similarity with CMPVX, where X=160, 155, 150, or 114 of SEQ ID NO:1 SEQ ID NO:1 is provided. The expression enhancer may further comprise a stuffer sequence fused to the 3′ end of the 5′UTR nucleotide sequence (CMPVX+, where X=160, 155, 150, or 114 of SEQ ID NO:1). The stuffer sequence may comprise one or more plant kozak sequences. Plants comprising the expression enhancer and methods using the expression enhancer are also described
Claims19
A nucleic acid molecule comprising:
- a first portion consisting of nucleotides 1-160 of SEQ ID NO:1 having a substitution of G115A,
- a second portion comprising a plant Kozak sequence, a multiple cloning site, or a combination of a plant Kozak sequence and a multiple cloning site, the second portion positioned 3′ to the first portion, and
- a third portion encoding a heterologous protein of interest, the third portion positioned 3′ to the second portion,
- wherein the nucleic acid molecule does not comprise nucleotides 161-509of SEQ ID NO:4.
Dependencies are inferred from the claim text.