Signal boost cascade assay
AssigneeVedaBio, Inc.
InventorsAnurup Ganguli, Ashish Pandey, Ariana Mostafa, Jacob Berger
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Abstract
The present disclosure relates to compositions of matter and assay methods used to detect one or more target nucleic acids of interest in a sample. The compositions and methods provide signal boost upon detection of target nucleic acids of interest in less than one minute and in some instances instantaneously at ambient temperatures down to 16° C. or less, without amplification of the target nucleic acids yet allowing for massive multiplexing, high accuracy and minimal non-specific signal generation.
Claims5
A method for preventing unwinding of blocked nucleic acid molecules in the presence of an RNP comprising the steps of:
- providing blocked nucleic acid molecules;
- providing ribonucleoprotein complexes comprising a Cas12a nucleic acid-guided nuclease that exhibits both cis- and trans-cleavage activity upon activation and a gRNA that recognizes an unblocked nucleic acid molecule resulting from trans-cleavage of the blocked nucleic acid molecules;
- and
- engineering the nucleic acid-guided nuclease used in the ribonucleoprotein complex to result in a variant nucleic acid-guided nuclease where single stranded DNA is cleaved faster than double stranded DNA is cleaved.
Dependencies are inferred from the claim text.