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US 11,884,918 B2Granted
Systems and methods for modulating CRISPR activity
AssigneeSynthego Corporation
InventorsReed Kelso, Jared Carlson-Stevermer, Sahil Joshi, Travis Maures, Anastasia Kadina, John Andrew Walker, II
Life of this patent
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Abstract
Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides.
Claims9
A CRISPR OFF polynucleotide comprising:
- (a) a guide sequence configured to anneal to a target sequence in a target nucleic acid molecule;
- (b) a sequence configured to bind to a CRISPR enzyme;
- and
- (c) a cleavable linker positioned at position 57 and/or 74 in the sequence configured to bind to the CRISPR enzyme wherein position 1 is at a 5′ end of the guide sequence and positions are counted from 5′ to 3′, and wherein the CRISPR OFF polynucleotide is inactivated when the cleavable linker is cleaved.
1 more element
Dependencies are inferred from the claim text.