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US 11,884,907 B2Granted
Culture container, method for culturing lymphocytes, culture-container production method, and solid-phasing apparatus
AssigneeToyo Seikan Group Holdings, Ltd.
InventorsTakahiko Totani, Satoshi Tanaka, Takeshi Aihara, Yoichi Ishizaki
Life of this patent
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Abstract
A culture container for culturing lymphocytes includes an immobilized surface and a non-immobilized surface, wherein the culture container is formed of a gas permeable film, the immobilized surface and the non-immobilized surface are container inner surfaces facing each other, and anti-CD3 antibodies are immobilized in the immobilized surface at a concentration of 10 to 300 ng/cm2.
Claims1
A culture bag for culturing lymphocytes, comprising:
- a first film member having gas permeability required for cell culture;
- and
- a second film member having gas permeability required for cell culture;
- wherein the first film member and the second film member are formed into bag-shape;
- a first surface inside the bag of the first film member is formed as an immobilized activating surface by immobilizing anti-CD3 antibodies at a concentration of 10 to 300 ng/cm2;
- a second surface inside the bag of the second film member is formed as a non-immobilized proliferating surface without immobilizing anti-CD3 antibodies;
- and
- a gas is sealed in an amount of 0.01 to 4 ml per cm2 of a bottom surface area in the culture bag.
5 more elements
Dependencies are inferred from the claim text.