US 11,884,905 B2 · Fluidic chip for cell culture use, culture vessel, and culture method

Abstract

The present invention provides a fluidic chip for cell culture use which can prevent a decrease in the activity of cultured cells in a preparation stage, and which makes it possible to observe a cultured cell tissue while detaching the cultured cell tissue from the fluidic chip. The fluidic chip according to the present invention is characterized by being provided with a base and a lid member, wherein the base and the lid member are configured in such a manner that a first flow path and a first accommodation section for attachably/detachably accommodating a first culture vessel are formed between the base and the lid member when the base and the lid member are bonded to each other, the first culture vessel encloses a culture gel into which cells or a cell tissue is to be embedded and is at least partially formed from a hydrogel or a porous body, and the base and the lid member are configured in such a manner that at least a portion of a culture solution flowing through the first flow path is supplied to the first accommodation section.

Claims11

Dependent claims

A fluidic chip for cell culture use, comprising a base and a lid member, wherein

the base and the lid member are configured to define one or both of a first flow path and a first accommodation section for attachably/detachably accommodating a first culture vessel when the lid member is joined to the base,
wherein the first culture vessel comprises a culture gel having cells or a cell tissue embedded in the culture gel and includes a light-permeable window made from a hydrogel or a porous body,
wherein the light-permeable window made from the hydrogel has a gel strength of from about 50 g/cm2 to about 10000 g/cm2,
the first accommodation section has a socket with which the first culture vessel is engageable, and
the base and the lid member are configured in such a manner that at least a portion of a culture solution flowing through the first flow path is supplied to the first accommodation section;
further wherein the hydrogel is selected from the group consisting of:
an agarose gel, a polyacrylamide gel, a sodium alginate, and a collagen gel.

4 more elements

Dependencies are inferred from the claim text.

Fluidic chip for cell culture use, culture vessel, and culture method

Life of this patent

Fluidic chip for cell culture use, culture vessel, and culture method

Life of this patent

Abstract

Claims11