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AssigneeZhejiang University
InventorsYirong Guo, Yunyun Chang, Rubing Zou, Ying Zhao, Shasha Jiao, Guonian Zhu
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A variable region sequence of a specific antibody against clothianidin is provided. A gene encoding a heavy chain variable region of the antibody has an amino acid sequence shown in SEQ ID NO: 2. An intact recombinant antibody against clothianidin is provided. The intact recombinant antibody includes a heavy chain constant region, a heavy chain variable region, a light chain constant region and a light chain variable region, wherein a gene encoding the heavy chain variable region has an amino acid sequence shown in SEQ ID NO: 2. An immunostrip containing the antibody for rapid detection of clothianidin residue is also provided. The sequence genes obtained are linked to an expression vector containing a heavy chain constant region gene and a light chain constant region gene, respectively, and an intact recombinant antibody is expressed and obtained by using mammalian cells with a double-plasmid system.
An intact recombinant antibody against clothianidin, comprising a heavy chain constant region, a heavy chain variable region, a light chain constant region, and a light chain variable region, wherein the amino acid sequence of the heavy chain variable region comprises SEQ ID NO:
An expression plasmid for expressing an intact recombinant antibody against clothianidin, comprising:
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