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US 11,884,741 B2Granted
Method for improving thermal stability of antibody and method for producing modified antibody
AssigneeSysmex Corporation
InventorsTomofumi Nakada, Nobuyuki Ide
Life of this patent
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Abstract
Disclosed is a method for producing a modified antibody, comprising: in a heavy chain of the antibody, changing at least one amino acid residue selected from the group consisting of 8th to 11th amino acid residues based on Kabat method to a cysteine residue, and changing at least one amino acid residue selected from the group consisting of 109th and 110th amino acid residues based on IMGT method to a cysteine residue; and recovering the modified antibody.
Claims17
A method for improving thermal stability of an antibody, comprising:
- in a heavy chain of the antibody, changing at least one amino acid residue selected from the group consisting of 8th to 11th amino acid residues based on Kabat method to a cysteine residue, and changing at least one amino acid residue selected from the group consisting of 109th and 110th amino acid residues based on IMGT method to a cysteine residue,
- wherein the modified antibody has improved thermal stability as compared to the unmodified antibody;
- and
- wherein the modified antibody is Fab, Fab′, F(ab′)2, reduced IgG, IgG, IgA, IgM, IgD or IgE.
1 more element
A method for producing a modified antibody, comprising:
- in a heavy chain of the antibody, changing at least one amino acid residue selected from the group consisting of 8th to 11th amino acid residues based on Kabat method to a cysteine residue, and changing at least one amino acid residue selected from the group consisting of 109th and 110th amino acid residues based on IMGT method to a cysteine residue;
- wherein a disulfide bond is formed between the cysteine residue at any position of 8 to 11 based on the Kabat method and the cysteine residue at any position of 109 and 110 based on the IMGT method;
- wherein the modified antibody is Fab, Fab′, F(ab′)2, reduced IgG, IgG, IgA, IgM, IgD or IgE, and
- recovering the modified antibody.
1 more element
Dependencies are inferred from the claim text.