Immunoglobulin binding protein, and affinity support using same
AssigneesJsr Corporation, Jsr Life Sciences Corporation, JSR Micro N.V.
InventorsJun-ichi Yasuoka, Kiichi Yoshimura, Tomoaki Haga
Life of this patent
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Abstract
Provided are a protein L-derived immunoglobulin binding protein having an increased antibody dissociation rate under acidic conditions, and an affinity support using the same. Disclosed are an immunoglobulin binding protein comprising at least one mutant of an immunoglobulin binding domain, and an affinity support comprising a solid-phase support having the immunoglobulin binding protein bound thereto. A mutant of the immunoglobulin binding domain consists of an amino acid sequence having an identity of at least 85% with the sequence set forth in any one of SEQ ID NO:1 to SEQ ID NO:9 and a predetermined mutation, and the mutant has immunoglobulin κ chain binding activity.
Claims13
An immunoglobulin binding protein, comprising at least one mutant of an immunoglobulin binding domain,
- wherein the at least one mutant of the immunoglobulin binding domain consists of an amino acid sequence having an identity of at least 95% with the sequence set forth in SEQ NO:9, the amino acid sequence having substitution of lysine at a position corresponding to the 26th position of the amino acid sequence set forth in SEQ ID NO:9 with arginine, substitution of glycine at a position corresponding to the 34th position of the amino acid sequence set forth in SEQ ID NO:9 with histidine, and optionally at least one of substitution of threonine at a position corresponding to the 35th position of the amino acid sequence set forth in SEQ ID NO:9 with arginine, substitution of glycine at a position corresponding to the 42nd position of the amino acid sequence set forth in SEQ ID NO:9 with lysine, and substitution of asparagine at a position corresponding to the 54th position of the amino acid sequence set forth in SEQ ID NO:9 with glutamine.
A method for producing a mutant of an immunoglobulin binding domain, the method comprising:
- providing a polypeptide consisting of the amino acid sequence set forth in SEQ ID NO:
- 9 or an amino acid sequence having an identity of at least 95% therewith,
- introducing into the polypeptide at least one mutation selected from the group consisting of substitution of lysine at a position corresponding to the 26th position of the amino acid sequence set forth in SEQ ID NO:9 with arginine, substitution of glycine at a position corresponding to the 34th position of the amino acid sequence set forth in SEQ ID NO:9 with histidine, and optionally at least one of substitution of threonine at a position corresponding to the 35th position of the amino acid sequence set forth in SEQ ID NO:9 with arginine, substitution of glycine at a position corresponding to the 42nd position of the amino acid sequence set forth in SEQ ID NO:9 with lysine, and substitution of asparagine at a position corresponding to the 54th position of the amino acid sequence set forth in SEQ ID NO:9 with glutamine, to produce a mutant immunoglobulin binding domain having immunoglobulin κ chain binding activity.
Dependencies are inferred from the claim text.