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US 11,884,700 B2Granted
Methods of inactivating viral contaminants
AssigneeIchnos Sciences SA
InventorsFilipa Abrantes, Sonia Letestu, Laure Cahuzac, Lionel Duarte
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Abstract
The present invention relates to a method for preparing an antibody-containing solution free of viral contaminants starting from cultured cells are described. The method include a step of subjecting the antibody containing solution to a mix of solvent and detergent or to high pH.
Claims14
A method for the removal of impurities from a clarified harvest antibody material, the method comprising:
- (a) culturing host cells which express an antibody, (b) harvesting the antibody material in the cell culture and (c) subjecting it to a clarification step, wherein said method further comprises (d) treating the resulting clarified harvest antibody material with high pH to reach a high target pH and (e) subjecting the high pH treated clarified harvest antibody material to a filtration step to remove impurities comprising host cell proteins, nucleic acids and/or cell debris wherein said high target pH is about 10.2 to about 11.0.
A method for the removal of impurities from a clarified harvest antibody material, the method comprising:
- (a) culturing host cells which express an antibody, (b) harvesting the antibody material in the cell culture and (c) subjecting it to a clarification step, wherein said method further comprises (d) subjecting said clarified harvest antibody material to protein A chromatography, (e) treating a resulting protein A eluate with high pH to reach a high target pH and (f) subjecting the high pH treated protein A eluate to a filtration step to remove impurities comprising host cell proteins, nucleic acids and/or cell debris, wherein said high target pH is about 10.2 to about 11.0.
Dependencies are inferred from the claim text.